![]() You are welcome to place the drain hose in the plug and leave it to thaw in your absence. Allow it to thaw and then drain and clean out condenser basin as necessary. ![]() Remove the metal shelving unit and manifold adapter plate to access the condenser basin. DO NOT SET THE DRUM WITH O-RING SIDE DOWN ON THE FLOOR. Invert drum manifold and carefully place on countertop so that the bottom lip is accessible. ![]() Do not lift using top valves as handles, THEY WILL BREAK. Place hands firmly on opposing sides of drum manifold and angle to one side. Slowly turn one valve from top of dryer counter clockwise to vent dryer body (acrylic plastic drum manifold). Turn the vacuum and condenser OFF by pressing each respective button in turn. ![]() It is common to come back the next day, turn off vacuum, vent, only to find you need more time. If you are uncertain how long it will take write down when you will be checking your samples. Allow approximately 15 minutes to pass, then return to ensure the pressure inside has reached below 100 mTorr.įill out the sign-in sheet. As the pressure decreases, check to see that all valves on top are closed (you can use a finger to check for suction or you can listen for a hissing noise. IMPORTANT: Watch the vacuum pressure on the freeze dryer until the pressure reading is below 100 Torr, this should be a fairly rapid process. Turn on vacuum by pressing the 'vacuum' button. The vacuum pump does not want liquid water moving through it, so please be patient and wait. It is critical that you wait to start the vacuum pump until the condenser is below -40 ☌. The physical principle involved in lyophilization is sublimation, which is the direct passage from a solid to a gaseous state, without passing through the liquid state. Using manifold, batch, or bulk methods, freeze dryers dehydrate materials for optimum preservation and recovery. The freeze dryer (lyophilizer) is equipment that allows the drying of materials without the need to heat them. Your sample material could creep up or even explode out the top of a vial, so take the time to secure your material inside the vial. A freeze dryer or lyophylizer freezes material and then reduces the pressure and adds heat so that the frozen water is sublimated. You may need to use dry ice or liquid nitrogen to get your samples to freeze if they are rich in solutes.Ĭonsider placing paper towel or kim wipe on top of an open sample container secured with a rubber band. If your samples are particularly wet, freeze them before putting them in the freeze dryer and keep them frozen while waiting for condenser to reach -40 ☌. If you are using the tree, place the bottom acrylic plate specific for the tree on top of the condenser basin then place the tree with white o-ring on top of the plate. If you are going to use the drum, make sure the metal shelves are spaced appropriately for your samples. Production Freeze Dryers Lyophilizers Quanta Production Freeze Dryer8 sq ft to 300 sq ft (0.743 to 27.871 sqM) QuantaS Steam Sterilizable Freeze Dryers (SIP)Up to 250 sq ft (23. Look in the cabinet shown in the photo to find supplies. While you are waiting for the temperature to drop below -40 ☌, decide if you are using the drum or the tree. With a dry condenser basin and the drain hose removed, make sure the unit has power (power switch on back) and turn on the condenser by pressing the 'condenser' button. If the condenser has liquid water in it, see clean up section below. The condenser should be dry and free of water (liquid or solid) before starting a new session.
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